Automated facial feature evaluation system to prevent stress of head fixed mice

Anna Nasr, Gianna Rettinger, Huibert D. Mansvelder, Robert N.S. Sachdev, Matthew E. Larkum

Head fixation of rodents is a widely utilized and important technique that enables laboratories to measure brain activity during behavior, but head fixation can increase stress which affects both behavior and underlying brain activity, as well as animal welfa…